Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for POLR1B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = POLR1B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of POLR1B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:112,053,884–112,054,856 | 487.6 kb | Distal (>10kb) Multiome HiCAR | 492 | |
| chr2:112,254,532–112,255,517 | 287.4 kb | Distal (>10kb) Multiome | 915 | |
| chr2:112,275,156–112,276,567 | 266.2 kb | Distal (>10kb) Multiome | 1201 | |
| chr2:112,480,926–112,482,608 | 61.3 kb | Distal (>10kb) Multiome | 860 | |
| chr2:112,541,785–112,542,849 | 232 bp | At TSS Multiome | 1070 | |
| chr2:112,583,940–112,585,172 | 42.1 kb | Distal (>10kb) Multiome | 949 | |
| chr2:112,645,080–112,646,681 | 103.4 kb | Distal (>10kb) Multiome | 980 | |
| chr2:112,674,793–112,675,390 | 132.6 kb | Distal (>10kb) Multiome | 54 | |
| chr2:112,726,233–112,727,218 | 184.4 kb | Distal (>10kb) Multiome | 667 | |
| chr2:112,763,867–112,765,097 | 222.3 kb | Distal (>10kb) Multiome | 854 |
Genomic view of the POLR1B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.