This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
Transcription factors with Perturb-seq knockdown data for PKM. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PKM upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PKM, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr15:72,116,889–72,118,908 | 113.1 kb | Distal (>10kb) Multiome | 885 | |
| chr15:72,184,180–72,184,756 | 46.8 kb | Distal (>10kb) Multiome | 376 | |
| chr15:72,196,452–72,198,720 | 33.3 kb | Distal (>10kb) Multiome | 458 | |
| chr15:72,226,391–72,227,225 | 4.3 kb | Proximal (<10kb) Multiome | 515 | |
| chr15:72,228,657–72,229,174 | 2.0 kb | Proximal (<10kb) | 506 | |
| chr15:72,229,966–72,232,028 | 144 bp | At TSS Multiome | 1128 | |
| chr15:72,232,245–72,232,932 | 1.0 kb | Proximal (<10kb) | 294 | |
| chr15:72,237,488–72,237,719 | 6.3 kb | Proximal (<10kb) | 463 | |
| chr15:72,242,418–72,243,365 | 11.8 kb | Distal (>10kb) Multiome | 236 | |
| chr15:72,271,919–72,273,237 | 41.5 kb | Distal (>10kb) Multiome | 687 | |
| chr15:72,319,497–72,320,675 | 88.8 kb | Distal (>10kb) Multiome | 632 | |
| chr15:72,375,551–72,376,566 | 144.8 kb | Distal (>10kb) Multiome | 1074 | |
| chr15:72,473,670–72,475,497 | 243.0 kb | Distal (>10kb) Multiome | 1105 |
Genomic view of the PKM locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.