This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family are involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PITX1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PITX1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PITX1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:135,027,489–135,027,710 | 5.4 kb | Proximal (<10kb) | 81 | |
| chr5:135,027,861–135,028,205 | 4.9 kb | Proximal (<10kb) | 79 | |
| chr5:135,028,731–135,029,454 | 3.7 kb | Proximal (<10kb) | 166 | |
| chr5:135,030,853–135,031,806 | 1.3 kb | Proximal (<10kb) | 138 | |
| chr5:135,032,878–135,033,426 | at TSS | At TSS | 412 | |
| chr5:135,033,567–135,034,894 | 432 bp | At TSS | 630 | |
| chr5:135,039,338–135,041,162 | 6.2 kb | Proximal (<10kb) | 207 |
Genomic view of the PITX1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.