This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
Transcription factors with Perturb-seq knockdown data for PFKL. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PFKL upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PFKL, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr21:44,011,848–44,013,192 | 287.5 kb | Distal (>10kb) Multiome | 873 | |
| chr21:44,283,183–44,283,881 | 16.6 kb | Distal (>10kb) Multiome | 19 | |
| chr21:44,297,704–44,297,934 | 2.1 kb | Proximal (<10kb) | 332 | |
| chr21:44,299,485–44,300,902 | 98 bp | At TSS Multiome | 730 | |
| chr21:44,338,994–44,339,895 | 39.2 kb | Distal (>10kb) Multiome | 904 | |
| chr21:44,341,372–44,342,456 | 41.9 kb | Distal (>10kb) Multiome | 87 | |
| chr21:44,454,821–44,456,545 | 155.3 kb | Distal (>10kb) Multiome | 481 | |
| chr21:44,510,378–44,510,951 | 210.6 kb | Distal (>10kb) Multiome | 473 |
Genomic view of the PFKL locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.