This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. The encoded protein has been shown to form a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca(2+) signaling. Alternative splicing results in multiple transcript variants and a pseudogene has been identified on chromosome 1.[provided by RefSeq, May 2010]
Transcription factors with Perturb-seq knockdown data for PEF1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PEF1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PEF1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:31,372,771–31,374,054 | 271.2 kb | Distal (>10kb) Multiome | 474 | |
| chr1:31,375,901–31,376,374 | 268.6 kb | Distal (>10kb) Multiome | 20 | |
| chr1:31,394,503–31,395,142 | 250.0 kb | Distal (>10kb) Multiome | 592 | |
| chr1:31,412,821–31,414,256 | 231.7 kb | Distal (>10kb) Multiome | 550 | |
| chr1:31,548,778–31,549,765 | 95.7 kb | Distal (>10kb) Multiome | 641 | |
| chr1:31,617,603–31,618,426 | 26.7 kb | Distal (>10kb) Multiome | 345 | |
| chr1:31,644,333–31,645,606 | 117 bp | At TSS Multiome | 926 | |
| chr1:31,647,107–31,647,947 | 2.7 kb | Proximal (<10kb) Multiome | 49 | |
| chr1:31,703,931–31,704,503 | 59.3 kb | Distal (>10kb) Multiome | 217 | |
| chr1:31,714,302–31,716,109 | 70.4 kb | Distal (>10kb) Multiome | 759 | |
| chr1:31,760,521–31,761,442 | 116.0 kb | Distal (>10kb) Multiome | 317 | |
| chr1:31,762,648–31,763,343 | 117.9 kb | Distal (>10kb) Multiome | 402 | |
| chr1:31,764,083–31,764,672 | 119.5 kb | Distal (>10kb) Multiome | 263 | |
| chr1:31,769,677–31,770,397 | 125.3 kb | Distal (>10kb) Multiome | 185 | |
| chr1:31,776,109–31,776,832 | 131.7 kb | Distal (>10kb) Multiome | 86 | |
| chr1:31,788,126–31,789,694 | 143.8 kb | Distal (>10kb) Multiome | 713 | |
| chr1:31,815,736–31,816,251 | 171.2 kb | Distal (>10kb) Multiome | 666 | |
| chr1:31,937,587–31,938,944 | 293.6 kb | Distal (>10kb) Multiome | 1111 | |
| chr1:31,943,994–31,945,090 | 299.7 kb | Distal (>10kb) Multiome | 826 |
Genomic view of the PEF1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.