The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and carbon dioxide, and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 beta subunit. Mutations in this gene are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2012]
Transcription factors with Perturb-seq knockdown data for PDHB. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PDHB upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PDHB, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:58,177,450–58,178,460 | 255.9 kb | Distal (>10kb) Multiome | 551 | |
| chr3:58,187,606–58,188,058 | 245.9 kb | Distal (>10kb) Multiome | 377 | |
| chr3:58,217,189–58,217,783 | 216.4 kb | Distal (>10kb) Multiome | 545 | |
| chr3:58,222,267–58,222,780 | 211.3 kb | Distal (>10kb) Multiome | 115 | |
| chr3:58,236,918–58,238,686 | 196.2 kb | Distal (>10kb) Multiome | 727 | |
| chr3:58,305,855–58,306,746 | 127.5 kb | Distal (>10kb) Multiome | 875 | |
| chr3:58,332,558–58,333,709 | 100.9 kb | Distal (>10kb) Multiome | 705 | |
| chr3:58,433,383–58,434,404 | 30 bp | At TSS Multiome | 938 | |
| chr3:58,434,510–58,435,588 | 1.4 kb | Proximal (<10kb) Multiome | 226 | |
| chr3:58,437,328–58,437,636 | 3.5 kb | Proximal (<10kb) | 17 | |
| chr3:58,441,570–58,442,309 | 8.1 kb | Proximal (<10kb) Multiome | 65 | |
| chr3:58,450,170–58,450,919 | 16.8 kb | Distal (>10kb) Multiome | 20 | |
| chr3:58,463,546–58,463,974 | 30.0 kb | Distal (>10kb) Multiome | 479 | |
| chr3:58,470,966–58,471,873 | 37.7 kb | Distal (>10kb) Multiome | 742 | |
| chr3:58,491,060–58,492,581 | 58.1 kb | Distal (>10kb) Multiome | 631 | |
| chr3:58,585,026–58,585,766 | 151.7 kb | Distal (>10kb) Multiome | 401 | |
| chr3:58,586,723–58,587,275 | 153.2 kb | Distal (>10kb) Multiome | 131 | |
| chr3:58,629,982–58,630,563 | 196.4 kb | Distal (>10kb) Multiome | 197 | |
| chr3:58,640,649–58,641,138 | 207.0 kb | Distal (>10kb) Multiome | 288 |
Genomic view of the PDHB locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.