Transcription factors with Perturb-seq knockdown data for PCAT14. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PCAT14 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PCAT14, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:23,533,405–23,533,643 | 3.2 kb | Proximal (<10kb) | 207 | |
| chr22:23,536,147–23,536,672 | 208 bp | At TSS | 281 | |
| chr22:23,537,973–23,538,579 | 1.1 kb | Proximal (<10kb) | 90 | |
| chr22:23,539,467–23,539,869 | 2.6 kb | Proximal (<10kb) | 274 |
Genomic view of the PCAT14 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.