This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for P4HB. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = P4HB upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of P4HB, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:81,636,404–81,637,301 | 223.6 kb | Distal (>10kb) Multiome | 946 | |
| chr17:81,648,048–81,648,550 | 212.3 kb | Distal (>10kb) Multiome | 88 | |
| chr17:81,652,970–81,653,572 | 207.4 kb | Distal (>10kb) Multiome | 188 | |
| chr17:81,666,251–81,667,166 | 193.9 kb | Distal (>10kb) Multiome | 901 | |
| chr17:81,683,344–81,684,556 | 176.7 kb | Distal (>10kb) Multiome | 926 | |
| chr17:81,702,879–81,703,868 | 157.2 kb | Distal (>10kb) Multiome | 981 | |
| chr17:81,711,721–81,712,812 | 148.3 kb | Distal (>10kb) Multiome | 1056 | |
| chr17:81,832,473–81,833,976 | 27.3 kb | Distal (>10kb) Multiome | 1015 | |
| chr17:81,859,762–81,861,595 | 275 bp | At TSS Multiome | 1134 | |
| chr17:81,867,228–81,867,711 | 6.7 kb | Proximal (<10kb) | 680 | |
| chr17:81,867,733–81,868,293 | 7.2 kb | Proximal (<10kb) | 676 | |
| chr17:81,868,421–81,868,978 | 8.0 kb | Proximal (<10kb) Multiome | 577 | |
| chr17:81,869,000–81,872,289 | 10.7 kb | Distal (>10kb) Multiome | 1136 | |
| chr17:81,891,143–81,892,257 | 31.2 kb | Distal (>10kb) Multiome | 969 | |
| chr17:81,910,443–81,911,980 | 51.0 kb | Distal (>10kb) Multiome | 806 | |
| chr17:81,917,586–81,918,522 | 57.6 kb | Distal (>10kb) Multiome | 619 | |
| chr17:81,923,285–81,923,863 | 62.9 kb | Distal (>10kb) Multiome | 399 | |
| chr17:81,926,141–81,929,133 | 67.3 kb | Distal (>10kb) Multiome | 1034 | |
| chr17:81,936,855–81,937,682 | 76.8 kb | Distal (>10kb) Multiome | 854 | |
| chr17:81,959,299–81,962,169 | 99.2 kb | Distal (>10kb) Multiome | 805 | |
| chr17:81,966,804–81,968,359 | 107.5 kb | Distal (>10kb) Multiome | 908 | |
| chr17:81,976,595–81,978,312 | 117.1 kb | Distal (>10kb) Multiome | 1001 | |
| chr17:82,019,856–82,020,391 | 159.5 kb | Distal (>10kb) Multiome | 436 | |
| chr17:82,022,355–82,024,111 | 162.5 kb | Distal (>10kb) Multiome | 898 | |
| chr17:82,029,976–82,032,662 | 171.3 kb | Distal (>10kb) Multiome | 695 | |
| chr17:82,036,902–82,037,961 | 177.0 kb | Distal (>10kb) Multiome | 892 | |
| chr17:82,051,387–82,052,184 | 191.2 kb | Distal (>10kb) Multiome | 479 | |
| chr17:82,065,558–82,066,150 | 205.3 kb | Distal (>10kb) Multiome | 967 | |
| chr17:82,096,478–82,100,203 | 238.0 kb | Distal (>10kb) Multiome | 1085 | |
| chr17:82,103,334–82,104,356 | 243.0 kb | Distal (>10kb) Multiome | 672 |
Genomic view of the P4HB locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.