This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for P4HA2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = P4HA2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of P4HA2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:132,003,192–132,003,913 | 224.4 kb | Distal (>10kb) Multiome | 421 | |
| chr5:132,010,633–132,012,365 | 216.1 kb | Distal (>10kb) Multiome | 361 | |
| chr5:132,226,810–132,228,575 | 69 bp | At TSS Multiome | 725 | |
| chr5:132,256,858–132,258,371 | 29.7 kb | Distal (>10kb) Multiome | 338 | |
| chr5:132,293,061–132,295,661 | 66.3 kb | Distal (>10kb) Multiome | 1053 | |
| chr5:132,369,150–132,370,578 | 141.9 kb | Distal (>10kb) Multiome | 819 | |
| chr5:132,410,330–132,411,433 | 183.1 kb | Distal (>10kb) Multiome | 815 | |
| chr5:132,419,541–132,419,986 | 192.0 kb | Distal (>10kb) Multiome | 481 | |
| chr5:132,426,660–132,427,851 | 199.2 kb | Distal (>10kb) Multiome | 540 | |
| chr5:132,489,909–132,492,004 | 263.0 kb | Distal (>10kb) Multiome | 899 | |
| chr5:132,495,550–132,497,614 | 268.6 kb | Distal (>10kb) Multiome | 946 | |
| chr5:132,526,534–132,527,026 | 299.0 kb | Distal (>10kb) Multiome | 329 |
Genomic view of the P4HA2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.