The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Transcription factors with Perturb-seq knockdown data for ORC1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ORC1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ORC1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:52,141,669–52,142,926 | 262.3 kb | Distal (>10kb) Multiome | 839 | |
| chr1:52,240,504–52,241,012 | 163.6 kb | Distal (>10kb) Multiome | 110 | |
| chr1:52,365,194–52,367,305 | 38.1 kb | Distal (>10kb) Multiome | 969 | |
| chr1:52,368,430–52,369,016 | 35.8 kb | Distal (>10kb) Multiome | 340 | |
| chr1:52,396,940–52,397,252 | 7.2 kb | Proximal (<10kb) | 16 | |
| chr1:52,403,732–52,405,027 | 104 bp | At TSS Multiome | 789 | |
| chr1:52,552,164–52,554,055 | 149.0 kb | Distal (>10kb) Multiome | 955 | |
| chr1:52,602,249–52,602,924 | 198.1 kb | Distal (>10kb) Multiome | 267 | |
| chr1:52,632,811–52,633,791 | 228.9 kb | Distal (>10kb) Multiome | 269 | |
| chr1:52,662,669–52,663,405 | 258.6 kb | Distal (>10kb) Multiome | 285 | |
| chr1:52,697,629–52,698,936 | 294.0 kb | Distal (>10kb) Multiome HiCAR | 997 | |
| chr1:52,702,879–52,703,689 | 298.7 kb | Distal (>10kb) Multiome | 872 |
Genomic view of the ORC1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.