The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for NUP50. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NUP50 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NUP50, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:45,006,916–45,010,513 | 153.8 kb | Distal (>10kb) Multiome | 522 | |
| chr22:45,052,140–45,053,598 | 111.0 kb | Distal (>10kb) Multiome | 132 | |
| chr22:45,159,739–45,159,956 | 4.0 kb | Proximal (<10kb) | 252 | |
| chr22:45,163,246–45,164,928 | 54 bp | At TSS Multiome | 938 | |
| chr22:45,239,931–45,241,595 | 77.1 kb | Distal (>10kb) Multiome | 482 | |
| chr22:45,267,970–45,269,955 | 105.3 kb | Distal (>10kb) Multiome | 600 | |
| chr22:45,309,117–45,310,833 | 145.9 kb | Distal (>10kb) Multiome | 821 | |
| chr22:45,413,403–45,414,177 | 249.8 kb | Distal (>10kb) Multiome | 453 | |
| chr22:45,435,549–45,435,992 | 271.9 kb | Distal (>10kb) Multiome | 461 | |
| chr22:45,443,959–45,444,454 | 280.3 kb | Distal (>10kb) Multiome | 24 |
Genomic view of the NUP50 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.