This gene encodes a member of the netrin family of proteins, which function in various biological processes including axon guidance, tumorogenesis, and angiogenesis. Netrins are laminin-related proteins that have an N-terminal laminin-type domain, epidermal growth factor-like repeat domain, and a positively charged heparin-binding domain at the C-terminus. The protein encoded by this gene is involved in processes including neurite growth and migration, angiogenesis and mural cell adhesion to endothelial cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016]
Transcription factors with Perturb-seq knockdown data for NTN4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NTN4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NTN4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:95,547,928–95,549,389 | 242.0 kb | Distal (>10kb) Multiome | 471 | |
| chr12:95,551,266–95,551,780 | 239.2 kb | Distal (>10kb) Multiome | 171 | |
| chr12:95,745,876–95,746,868 | 44.4 kb | Distal (>10kb) Multiome | 68 | |
| chr12:95,789,276–95,791,638 | 228 bp | At TSS Multiome | 591 | |
| chr12:95,858,308–95,859,272 | 68.1 kb | Distal (>10kb) Multiome | 778 | |
| chr12:95,942,226–95,943,879 | 152.2 kb | Distal (>10kb) Multiome | 840 | |
| chr12:96,034,637–96,036,352 | 244.9 kb | Distal (>10kb) Multiome | 921 |
Genomic view of the NTN4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.