This gene encodes a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The encoded protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene. There is a pseudogene of the 3' UTR region of this gene on chromosome X. [provided by RefSeq, Jul 2014]
Transcription factors with Perturb-seq knockdown data for NSL1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NSL1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NSL1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:212,498,151–212,499,452 | 292.9 kb | Distal (>10kb) Multiome | 216 | |
| chr1:212,514,035–212,515,926 | 276.3 kb | Distal (>10kb) Multiome | 524 | |
| chr1:212,558,067–212,559,384 | 232.8 kb | Distal (>10kb) Multiome | 891 | |
| chr1:212,605,830–212,610,097 | 182.1 kb | Distal (>10kb) Multiome | 1097 | |
| chr1:212,629,910–212,630,849 | 161.5 kb | Distal (>10kb) Multiome | 331 | |
| chr1:212,665,118–212,665,789 | 126.3 kb | Distal (>10kb) Multiome | 678 | |
| chr1:212,698,874–212,700,500 | 92.4 kb | Distal (>10kb) Multiome | 710 | |
| chr1:212,791,461–212,792,424 | 93 bp | At TSS Multiome | 1108 | |
| chr1:212,857,574–212,859,115 | 66.3 kb | Distal (>10kb) Multiome HiCAR | 946 | |
| chr1:212,860,779–212,861,295 | 69.2 kb | Distal (>10kb) Multiome | 315 | |
| chr1:212,907,964–212,908,918 | 116.6 kb | Distal (>10kb) Multiome | 62 | |
| chr1:212,909,569–212,910,174 | 118.3 kb | Distal (>10kb) Multiome | 97 | |
| chr1:212,916,378–212,917,359 | 125.2 kb | Distal (>10kb) Multiome | 306 | |
| chr1:212,928,624–212,929,308 | 137.3 kb | Distal (>10kb) Multiome | 145 | |
| chr1:212,950,130–212,951,519 | 158.7 kb | Distal (>10kb) Multiome HiCAR | 457 | |
| chr1:212,967,458–212,968,096 | 176.1 kb | Distal (>10kb) Multiome | 178 | |
| chr1:213,015,122–213,015,983 | 223.9 kb | Distal (>10kb) Multiome | 888 | |
| chr1:213,050,888–213,051,797 | 259.5 kb | Distal (>10kb) Multiome | 880 | |
| chr1:213,066,260–213,067,034 | 274.9 kb | Distal (>10kb) Multiome | 70 |
Genomic view of the NSL1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.