This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described. [provided by RefSeq, May 2015]
Transcription factors with Perturb-seq knockdown data for NSD3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NSD3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NSD3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:38,104,797–38,106,255 | 276.6 kb | Distal (>10kb) Multiome | 954 | |
| chr8:38,108,727–38,109,662 | 273.2 kb | Distal (>10kb) Multiome | 83 | |
| chr8:38,175,948–38,177,501 | 205.5 kb | Distal (>10kb) Multiome | 870 | |
| chr8:38,230,935–38,232,445 | 150.7 kb | Distal (>10kb) Multiome | 904 | |
| chr8:38,268,159–38,269,673 | 113.0 kb | Distal (>10kb) Multiome | 795 | |
| chr8:38,381,367–38,383,496 | 102 bp | At TSS Multiome | 956 | |
| chr8:38,385,091–38,385,422 | 3.4 kb | Proximal (<10kb) | 278 | |
| chr8:38,385,791–38,387,334 | 4.2 kb | Proximal (<10kb) Multiome | 816 | |
| chr8:38,440,285–38,442,102 | 58.6 kb | Distal (>10kb) Multiome | 293 | |
| chr8:38,442,374–38,442,980 | 60.4 kb | Distal (>10kb) Multiome | 254 | |
| chr8:38,467,549–38,469,321 | 86.4 kb | Distal (>10kb) Multiome | 775 | |
| chr8:38,471,859–38,472,443 | 89.9 kb | Distal (>10kb) Multiome | 84 | |
| chr8:38,512,281–38,512,945 | 130.3 kb | Distal (>10kb) Multiome | 187 | |
| chr8:38,513,847–38,514,352 | 131.8 kb | Distal (>10kb) Multiome | 185 | |
| chr8:38,530,520–38,531,118 | 148.7 kb | Distal (>10kb) Multiome | 174 | |
| chr8:38,541,080–38,541,513 | 159.0 kb | Distal (>10kb) Multiome | 114 | |
| chr8:38,553,735–38,554,545 | 171.8 kb | Distal (>10kb) Multiome | 413 | |
| chr8:38,566,934–38,567,642 | 185.1 kb | Distal (>10kb) Multiome | 337 | |
| chr8:38,734,447–38,735,152 | 352.6 kb | Distal (>10kb) Multiome HiCAR | 738 |
Genomic view of the NSD3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.