Transcription factors with Perturb-seq knockdown data for NR2F1-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NR2F1-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NR2F1-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:93,412,609–93,413,070 | 9.4 kb | Proximal (<10kb) | 28 | |
| chr5:93,417,644–93,418,266 | 4.2 kb | Proximal (<10kb) | 23 | |
| chr5:93,421,447–93,421,820 | 602 bp | At TSS | 94 | |
| chr5:93,422,023–93,422,556 | at TSS | At TSS | 85 | |
| chr5:93,422,676–93,423,500 | 253 bp | At TSS | 104 | |
| chr5:93,456,680–93,457,624 | 34.7 kb | Distal (>10kb) Multiome | 88 | |
| chr5:93,563,617–93,564,212 | 141.6 kb | Distal (>10kb) Multiome | 95 | |
| chr5:93,569,362–93,571,779 | 148.5 kb | Distal (>10kb) Multiome | 360 | |
| chr5:93,578,889–93,582,135 | 159.1 kb | Distal (>10kb) Multiome | 840 | |
| chr5:93,596,297–93,597,643 | 174.8 kb | Distal (>10kb) Multiome | 351 | |
| chr5:93,620,145–93,621,850 | 198.9 kb | Distal (>10kb) Multiome | 782 |
Genomic view of the NR2F1-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.