Enables DNA-binding transcription factor binding activity; protein serine/threonine kinase activity; and ubiquitin protein ligase binding activity. Involved in several processes, including negative regulation of TORC1 signaling; protein stabilization; and transforming growth factor beta receptor signaling pathway. Predicted to be located in cytosol and nucleoplasm. Predicted to be active in cytoplasm and nucleus. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for NLK. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NLK upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NLK, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:27,892,882–27,893,663 | 148.4 kb | Distal (>10kb) Multiome | 607 | |
| chr17:27,915,662–27,916,230 | 125.9 kb | Distal (>10kb) Multiome | 185 | |
| chr17:27,939,884–27,941,043 | 101.5 kb | Distal (>10kb) Multiome | 80 | |
| chr17:27,982,605–27,983,118 | 58.9 kb | Distal (>10kb) Multiome | 124 | |
| chr17:28,040,846–28,042,232 | 242 bp | At TSS Multiome | 682 | |
| chr17:28,227,184–28,228,835 | 185.9 kb | Distal (>10kb) Multiome | 426 | |
| chr17:28,251,169–28,251,939 | 209.8 kb | Distal (>10kb) Multiome | 480 | |
| chr17:28,317,975–28,319,646 | 277.3 kb | Distal (>10kb) Multiome | 858 | |
| chr17:28,321,010–28,321,937 | 279.7 kb | Distal (>10kb) Multiome | 25 | |
| chr17:28,335,024–28,336,772 | 293.9 kb | Distal (>10kb) Multiome | 933 |
Genomic view of the NLK locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.