This gene encodes a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. The encoded protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of multiple mitochondrial dysfunctions syndrome-1, and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
Transcription factors with Perturb-seq knockdown data for NFU1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NFU1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NFU1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:69,178,030–69,178,850 | 259.0 kb | Distal (>10kb) Multiome | 832 | |
| chr2:69,306,399–69,307,697 | 130.1 kb | Distal (>10kb) Multiome | 707 | |
| chr2:69,348,684–69,349,416 | 88.4 kb | Distal (>10kb) Multiome | 41 | |
| chr2:69,386,703–69,387,800 | 50.2 kb | Distal (>10kb) Multiome | 995 | |
| chr2:69,435,560–69,435,948 | 1.5 kb | Proximal (<10kb) | 24 | |
| chr2:69,436,764–69,438,011 | 28 bp | At TSS Multiome | 972 | |
| chr2:69,450,768–69,451,516 | 13.7 kb | Distal (>10kb) Multiome | 69 | |
| chr2:69,642,875–69,644,362 | 206.4 kb | Distal (>10kb) Multiome | 960 |
Genomic view of the NFU1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.