This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
Transcription factors with Perturb-seq knockdown data for NFKBIA. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NFKBIA upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NFKBIA, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:35,121,659–35,123,040 | 282.7 kb | Distal (>10kb) Multiome HiCAR | 1148 | |
| chr14:35,291,742–35,292,860 | 112.4 kb | Distal (>10kb) Multiome HiCAR | 994 | |
| chr14:35,336,559–35,337,059 | 67.8 kb | Distal (>10kb) Multiome | 773 | |
| chr14:35,341,371–35,342,254 | 62.9 kb | Distal (>10kb) Multiome HiCAR | 604 | |
| chr14:35,346,777–35,348,358 | 56.8 kb | Distal (>10kb) Multiome HiCAR | 829 | |
| chr14:35,355,863–35,357,022 | 48.4 kb | Distal (>10kb) Multiome | 595 | |
| chr14:35,397,579–35,397,954 | 6.7 kb | Proximal (<10kb) | 270 | |
| chr14:35,398,090–35,398,474 | 6.2 kb | Proximal (<10kb) | 148 | |
| chr14:35,398,720–35,399,769 | 4.9 kb | Proximal (<10kb) | 148 | |
| chr14:35,403,426–35,405,915 | 161 bp | At TSS Multiome | 1102 | |
| chr14:35,407,054–35,407,230 | 2.4 kb | Proximal (<10kb) | 262 | |
| chr14:35,413,070–35,413,554 | 8.4 kb | Proximal (<10kb) | 382 | |
| chr14:35,533,170–35,535,985 | 129.3 kb | Distal (>10kb) Multiome | 1111 |
Genomic view of the NFKBIA locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.