This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]
Transcription factors with Perturb-seq knockdown data for NEK2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NEK2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NEK2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:211,375,133–211,375,818 | 300.2 kb | Distal (>10kb) Multiome | 107 | |
| chr1:211,382,531–211,383,307 | 292.8 kb | Distal (>10kb) Multiome | 921 | |
| chr1:211,398,188–211,399,458 | 276.8 kb | Distal (>10kb) Multiome | 128 | |
| chr1:211,399,597–211,400,219 | 275.5 kb | Distal (>10kb) Multiome | 80 | |
| chr1:211,416,272–211,417,162 | 259.0 kb | Distal (>10kb) Multiome | 257 | |
| chr1:211,469,768–211,470,490 | 205.4 kb | Distal (>10kb) Multiome | 383 | |
| chr1:211,513,158–211,514,512 | 161.5 kb | Distal (>10kb) Multiome | 484 | |
| chr1:211,515,159–211,516,023 | 160.0 kb | Distal (>10kb) Multiome | 818 | |
| chr1:211,577,546–211,580,066 | 96.2 kb | Distal (>10kb) Multiome | 1012 | |
| chr1:211,630,152–211,630,733 | 45.2 kb | Distal (>10kb) Multiome | 515 | |
| chr1:211,655,045–211,655,900 | 20.2 kb | Distal (>10kb) Multiome | 351 | |
| chr1:211,667,030–211,667,875 | 8.2 kb | Proximal (<10kb) Multiome | 301 | |
| chr1:211,675,193–211,676,330 | 173 bp | At TSS Multiome | 936 | |
| chr1:211,680,790–211,681,020 | 5.2 kb | Proximal (<10kb) | 287 | |
| chr1:211,681,130–211,681,410 | 5.5 kb | Proximal (<10kb) | 154 | |
| chr1:211,681,537–211,681,820 | 5.9 kb | Proximal (<10kb) | 22 | |
| chr1:211,695,364–211,695,890 | 20.1 kb | Distal (>10kb) Multiome | 283 | |
| chr1:211,698,066–211,699,596 | 23.0 kb | Distal (>10kb) Multiome | 651 | |
| chr1:211,829,926–211,831,171 | 155.1 kb | Distal (>10kb) Multiome HiCAR | 682 | |
| chr1:211,889,249–211,890,017 | 214.0 kb | Distal (>10kb) Multiome | 139 | |
| chr1:211,895,246–211,895,795 | 219.9 kb | Distal (>10kb) Multiome | 267 | |
| chr1:212,034,569–212,036,407 | 359.8 kb | Distal (>10kb) Multiome HiCAR | 995 | |
| chr1:212,309,881–212,310,794 | 634.6 kb | Distal (>10kb) Multiome HiCAR | 183 | |
| chr1:212,315,914–212,316,445 | 640.5 kb | Distal (>10kb) Multiome HiCAR | 140 | |
| chr1:212,413,972–212,416,004 | 739.3 kb | Distal (>10kb) Multiome HiCAR | 833 |
Genomic view of the NEK2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.