This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. The encoded protein catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to nitrogen of glucosamine in heparan sulfate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Transcription factors with Perturb-seq knockdown data for NDST1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NDST1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NDST1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:150,211,110–150,211,964 | 274.3 kb | Distal (>10kb) Multiome | 131 | |
| chr5:150,302,023–150,303,433 | 183.0 kb | Distal (>10kb) Multiome | 377 | |
| chr5:150,357,249–150,358,378 | 128.2 kb | Distal (>10kb) Multiome | 746 | |
| chr5:150,409,825–150,410,350 | 75.8 kb | Distal (>10kb) Multiome | 299 | |
| chr5:150,412,530–150,413,063 | 73.0 kb | Distal (>10kb) Multiome | 495 | |
| chr5:150,441,289–150,442,097 | 44.2 kb | Distal (>10kb) Multiome | 188 | |
| chr5:150,449,356–150,450,212 | 35.9 kb | Distal (>10kb) Multiome | 1068 | |
| chr5:150,458,741–150,459,365 | 26.8 kb | Distal (>10kb) Multiome | 430 | |
| chr5:150,485,401–150,487,150 | 30 bp | At TSS Multiome | 761 | |
| chr5:150,491,951–150,492,394 | 6.1 kb | Proximal (<10kb) | 25 | |
| chr5:150,577,977–150,578,481 | 92.5 kb | Distal (>10kb) Multiome | 149 | |
| chr5:150,624,424–150,625,067 | 139.0 kb | Distal (>10kb) Multiome | 250 | |
| chr5:150,656,143–150,657,405 | 170.9 kb | Distal (>10kb) Multiome | 607 | |
| chr5:150,670,030–150,670,981 | 184.8 kb | Distal (>10kb) Multiome | 69 | |
| chr5:150,671,571–150,672,919 | 186.6 kb | Distal (>10kb) Multiome | 303 | |
| chr5:150,700,548–150,701,464 | 215.2 kb | Distal (>10kb) Multiome | 858 | |
| chr5:150,758,281–150,759,438 | 273.0 kb | Distal (>10kb) Multiome | 902 | |
| chr5:150,778,240–150,779,492 | 293.2 kb | Distal (>10kb) Multiome | 723 | |
| chr5:150,783,348–150,783,999 | 297.8 kb | Distal (>10kb) Multiome | 435 |
Genomic view of the NDST1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.