This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for NCK2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NCK2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NCK2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:105,736,265–105,736,695 | 8.4 kb | Proximal (<10kb) | 19 | |
| chr2:105,736,844–105,736,973 | 8.1 kb | Proximal (<10kb) | 11 | |
| chr2:105,744,347–105,745,826 | 230 bp | At TSS Multiome | 530 | |
| chr2:105,745,948–105,746,797 | 1.3 kb | Proximal (<10kb) Multiome | 250 | |
| chr2:105,849,142–105,850,030 | 104.5 kb | Distal (>10kb) Multiome | 70 | |
| chr2:105,950,444–105,951,318 | 205.9 kb | Distal (>10kb) Multiome HiCAR | 203 |
Genomic view of the NCK2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.