Nicotinic acid (NA; niacin) is converted by nicotinic acid phosphoribosyltransferase (NAPRT; EC 2.4.2.11) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular redox reactions and is an essential component of a variety of processes in cellular metabolism including response to stress (Hara et al., 2007).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for NAPRT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NAPRT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NAPRT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:143,290,813–143,291,709 | 287.0 kb | Distal (>10kb) Multiome | 909 | |
| chr8:143,334,216–143,335,267 | 243.5 kb | Distal (>10kb) Multiome | 688 | |
| chr8:143,368,147–143,369,893 | 209.6 kb | Distal (>10kb) Multiome | 698 | |
| chr8:143,407,212–143,408,612 | 170.2 kb | Distal (>10kb) Multiome | 447 | |
| chr8:143,428,968–143,431,754 | 147.4 kb | Distal (>10kb) Multiome | 743 | |
| chr8:143,540,714–143,542,061 | 36.8 kb | Distal (>10kb) Multiome | 849 | |
| chr8:143,553,122–143,554,177 | 24.6 kb | Distal (>10kb) Multiome | 775 | |
| chr8:143,558,225–143,558,936 | 19.8 kb | Distal (>10kb) Multiome | 600 | |
| chr8:143,567,166–143,570,227 | 9.2 kb | Proximal (<10kb) Multiome | 485 | |
| chr8:143,571,367–143,572,283 | 6.6 kb | Proximal (<10kb) Multiome | 443 | |
| chr8:143,577,641–143,578,799 | 46 bp | At TSS Multiome | 470 | |
| chr8:143,596,744–143,599,643 | 19.3 kb | Distal (>10kb) Multiome | 973 | |
| chr8:143,608,979–143,610,357 | 31.4 kb | Distal (>10kb) Multiome | 735 | |
| chr8:143,617,068–143,618,380 | 39.3 kb | Distal (>10kb) Multiome | 823 | |
| chr8:143,635,715–143,636,601 | 57.7 kb | Distal (>10kb) Multiome | 786 | |
| chr8:143,684,308–143,684,747 | 106.1 kb | Distal (>10kb) Multiome | 686 | |
| chr8:143,716,075–143,716,913 | 138.0 kb | Distal (>10kb) Multiome | 311 | |
| chr8:143,733,345–143,734,440 | 155.5 kb | Distal (>10kb) Multiome | 663 | |
| chr8:143,740,039–143,740,754 | 162.2 kb | Distal (>10kb) Multiome | 308 | |
| chr8:143,771,202–143,772,221 | 193.5 kb | Distal (>10kb) Multiome | 405 | |
| chr8:143,814,945–143,816,493 | 237.4 kb | Distal (>10kb) Multiome | 893 | |
| chr8:143,828,724–143,829,946 | 251.0 kb | Distal (>10kb) Multiome | 933 | |
| chr8:143,840,284–143,841,979 | 262.8 kb | Distal (>10kb) Multiome | 551 | |
| chr8:143,877,814–143,879,346 | 300.2 kb | Distal (>10kb) Multiome | 444 |
Genomic view of the NAPRT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.