This gene encodes one of the myosin light chains, a component of the hexameric ATPase cellular motor protein myosin. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene product, one of the regulatory light chains, is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MYL5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MYL5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MYL5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:473,613–474,432 | 199.4 kb | Distal (>10kb) Multiome | 745 | |
| chr4:498,731–499,543 | 174.4 kb | Distal (>10kb) Multiome | 869 | |
| chr4:575,294–576,132 | 98.1 kb | Distal (>10kb) Multiome | 154 | |
| chr4:663,207–664,324 | 9.8 kb | Proximal (<10kb) Multiome | 298 | |
| chr4:673,501–675,336 | 823 bp | At TSS Multiome | 920 | |
| chr4:680,919–681,550 | 7.8 kb | Proximal (<10kb) Multiome HiCAR | 275 | |
| chr4:684,015–684,449 | 9.5 kb | Proximal (<10kb) | 423 | |
| chr4:688,280–689,636 | 15.7 kb | Distal (>10kb) Multiome HiCAR | 218 | |
| chr4:705,313–706,398 | 32.1 kb | Distal (>10kb) Multiome | 613 | |
| chr4:781,187–782,732 | 108.4 kb | Distal (>10kb) Multiome | 893 | |
| chr4:825,232–826,278 | 152.2 kb | Distal (>10kb) Multiome | 317 | |
| chr4:931,599–932,710 | 258.8 kb | Distal (>10kb) Multiome | 818 | |
| chr4:945,351–946,054 | 272.0 kb | Distal (>10kb) Multiome | 73 | |
| chr4:972,792–974,408 | 300.0 kb | Distal (>10kb) Multiome | 437 |
Genomic view of the MYL5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.