This gene encodes a structure-specific endonuclease which belongs to the XPF/MUS81 endonuclease family and plays a critical role in the resolution of recombination intermediates during DNA repair after inter-strand cross-links, replication fork collapse, and DNA double-strand breaks. The encoded protein associates with one of two closely related essential meiotic endonuclease proteins (EME1 or EME2) to form a complex that processes DNA secondary structures. It contains an N-terminal DEAH helicase domain, an excision repair cross complementation group 4 (ERCC4) endonuclease domain, and two tandem C-terminal helix-hairpin-helix domains. Mice with a homozygous knockout of the orthologous gene have significant meiotic defects including the failure to repair a subset of DNA double strand breaks. [provided by RefSeq, Jun 2017]
Transcription factors with Perturb-seq knockdown data for MUS81. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MUS81 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MUS81, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:65,569,718–65,570,745 | 290.1 kb | Distal (>10kb) Multiome | 930 | |
| chr11:65,571,973–65,573,008 | 287.9 kb | Distal (>10kb) Multiome | 420 | |
| chr11:65,573,788–65,575,269 | 286.1 kb | Distal (>10kb) Multiome | 742 | |
| chr11:65,575,601–65,576,322 | 284.4 kb | Distal (>10kb) Multiome | 527 | |
| chr11:65,591,808–65,592,698 | 268.2 kb | Distal (>10kb) Multiome | 169 | |
| chr11:65,606,536–65,607,917 | 253.3 kb | Distal (>10kb) Multiome | 376 | |
| chr11:65,613,478–65,614,645 | 246.2 kb | Distal (>10kb) Multiome | 702 | |
| chr11:65,614,954–65,616,793 | 244.9 kb | Distal (>10kb) Multiome | 830 | |
| chr11:65,637,768–65,638,920 | 222.3 kb | Distal (>10kb) Multiome | 740 | |
| chr11:65,641,918–65,642,633 | 218.3 kb | Distal (>10kb) Multiome | 155 | |
| chr11:65,646,814–65,647,818 | 213.1 kb | Distal (>10kb) Multiome | 270 | |
| chr11:65,652,106–65,653,317 | 207.7 kb | Distal (>10kb) Multiome | 901 | |
| chr11:65,662,517–65,663,930 | 197.4 kb | Distal (>10kb) Multiome | 839 | |
| chr11:65,711,437–65,712,942 | 148.2 kb | Distal (>10kb) Multiome | 1143 | |
| chr11:65,720,215–65,720,955 | 139.8 kb | Distal (>10kb) Multiome | 821 | |
| chr11:65,779,808–65,781,988 | 80.4 kb | Distal (>10kb) Multiome | 764 | |
| chr11:65,785,898–65,789,101 | 72.0 kb | Distal (>10kb) Multiome | 478 | |
| chr11:65,817,968–65,818,502 | 42.2 kb | Distal (>10kb) Multiome | 694 | |
| chr11:65,833,325–65,834,189 | 26.6 kb | Distal (>10kb) Multiome | 396 | |
| chr11:65,856,804–65,858,748 | 3.4 kb | Proximal (<10kb) Multiome | 972 | |
| chr11:65,859,034–65,861,240 | 184 bp | At TSS Multiome | 1012 | |
| chr11:65,871,920–65,874,072 | 13.3 kb | Distal (>10kb) Multiome | 741 | |
| chr11:65,887,987–65,889,162 | 28.1 kb | Distal (>10kb) Multiome | 824 | |
| chr11:65,890,253–65,891,457 | 30.2 kb | Distal (>10kb) Multiome | 895 | |
| chr11:65,899,867–65,900,779 | 40.1 kb | Distal (>10kb) Multiome | 740 | |
| chr11:65,918,507–65,920,266 | 58.8 kb | Distal (>10kb) Multiome | 992 | |
| chr11:65,961,271–65,962,172 | 101.2 kb | Distal (>10kb) Multiome | 834 | |
| chr11:66,001,882–66,002,962 | 142.0 kb | Distal (>10kb) Multiome | 1022 | |
| chr11:66,007,227–66,007,703 | 147.1 kb | Distal (>10kb) Multiome | 100 | |
| chr11:66,022,341–66,022,903 | 162.2 kb | Distal (>10kb) Multiome | 466 | |
| chr11:66,045,170–66,045,637 | 184.9 kb | Distal (>10kb) Multiome | 394 | |
| chr11:66,048,912–66,049,460 | 188.7 kb | Distal (>10kb) Multiome | 83 | |
| chr11:66,051,716–66,052,760 | 191.8 kb | Distal (>10kb) Multiome | 901 | |
| chr11:66,069,690–66,071,102 | 209.7 kb | Distal (>10kb) Multiome | 622 |
Genomic view of the MUS81 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.