Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MRPL12. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MRPL12 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MRPL12, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:81,481,258–81,481,865 | 221.8 kb | Distal (>10kb) Multiome | 348 | |
| chr17:81,483,927–81,484,829 | 218.9 kb | Distal (>10kb) Multiome | 828 | |
| chr17:81,487,690–81,488,371 | 215.4 kb | Distal (>10kb) Multiome | 721 | |
| chr17:81,511,887–81,515,656 | 190.4 kb | Distal (>10kb) Multiome | 1116 | |
| chr17:81,518,632–81,519,878 | 183.8 kb | Distal (>10kb) Multiome | 617 | |
| chr17:81,537,462–81,537,907 | 165.7 kb | Distal (>10kb) Multiome | 282 | |
| chr17:81,551,821–81,553,314 | 150.2 kb | Distal (>10kb) Multiome | 845 | |
| chr17:81,636,404–81,637,301 | 66.3 kb | Distal (>10kb) Multiome | 946 | |
| chr17:81,648,048–81,648,550 | 55.1 kb | Distal (>10kb) Multiome | 88 | |
| chr17:81,652,970–81,653,572 | 50.2 kb | Distal (>10kb) Multiome | 188 | |
| chr17:81,666,251–81,667,166 | 36.7 kb | Distal (>10kb) Multiome | 901 | |
| chr17:81,683,344–81,684,556 | 19.5 kb | Distal (>10kb) Multiome | 926 | |
| chr17:81,702,879–81,703,868 | 16 bp | At TSS Multiome | 981 | |
| chr17:81,711,721–81,712,812 | 8.9 kb | Proximal (<10kb) Multiome | 1056 | |
| chr17:81,832,473–81,833,976 | 129.9 kb | Distal (>10kb) Multiome | 1015 | |
| chr17:81,859,762–81,861,595 | 157.5 kb | Distal (>10kb) Multiome | 1134 | |
| chr17:81,868,421–81,868,978 | 165.2 kb | Distal (>10kb) Multiome | 577 | |
| chr17:81,869,000–81,872,289 | 167.9 kb | Distal (>10kb) Multiome | 1136 | |
| chr17:81,891,143–81,892,257 | 188.4 kb | Distal (>10kb) Multiome | 969 | |
| chr17:81,910,443–81,911,980 | 208.2 kb | Distal (>10kb) Multiome | 806 | |
| chr17:81,917,586–81,918,522 | 214.8 kb | Distal (>10kb) Multiome | 619 | |
| chr17:81,923,285–81,923,863 | 220.2 kb | Distal (>10kb) Multiome | 399 | |
| chr17:81,926,141–81,929,133 | 224.5 kb | Distal (>10kb) Multiome | 1034 | |
| chr17:81,936,855–81,937,682 | 234.0 kb | Distal (>10kb) Multiome | 854 | |
| chr17:81,959,299–81,962,169 | 256.4 kb | Distal (>10kb) Multiome | 805 | |
| chr17:81,966,804–81,968,359 | 264.7 kb | Distal (>10kb) Multiome | 908 | |
| chr17:81,976,595–81,978,312 | 274.3 kb | Distal (>10kb) Multiome | 1001 |
Genomic view of the MRPL12 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.