This gene encodes a member of the calcium/calmodulin-dependent protein kinases (CAMK) Ser/Thr protein kinase family, which belongs to the protein kinase superfamily. This protein contains conserved DLG (asp-leu-gly) and ENIL (glu-asn-ile-leu) motifs, and an N-terminal polybasic region which binds importin A and the translation factor scaffold protein eukaryotic initiation factor 4G (eIF4G). This protein is one of the downstream kinases activated by mitogen-activated protein (MAP) kinases. It phosphorylates the eukaryotic initiation factor 4E (eIF4E), thus playing important roles in the initiation of mRNA translation, oncogenic transformation and malignant cell proliferation. In addition to eIF4E, this protein also interacts with von Hippel-Lindau tumor suppressor (VHL), ring-box 1 (Rbx1) and Cullin2 (Cul2), which are all components of the CBC(VHL) ubiquitin ligase E3 complex. Multiple alternatively spliced transcript variants have been found, but the full-length nature and biological activity of only two variants are determined. These two variants encode distinct isoforms which differ in activity and regulation, and in subcellular localization. [provided by RefSeq, Aug 2011]
Transcription factors with Perturb-seq knockdown data for MKNK2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MKNK2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MKNK2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:1,811,638–1,813,347 | 238.6 kb | Distal (>10kb) Multiome | 782 | |
| chr19:1,826,853–1,827,379 | 224.0 kb | Distal (>10kb) Multiome | 193 | |
| chr19:1,848,048–1,848,953 | 202.6 kb | Distal (>10kb) Multiome | 819 | |
| chr19:1,851,701–1,852,216 | 199.3 kb | Distal (>10kb) Multiome | 115 | |
| chr19:1,855,082–1,856,620 | 195.8 kb | Distal (>10kb) Multiome | 476 | |
| chr19:1,861,439–1,865,723 | 187.3 kb | Distal (>10kb) Multiome | 891 | |
| chr19:1,875,645–1,876,693 | 175.0 kb | Distal (>10kb) Multiome | 887 | |
| chr19:1,885,058–1,885,861 | 165.7 kb | Distal (>10kb) Multiome | 495 | |
| chr19:1,904,556–1,906,084 | 145.8 kb | Distal (>10kb) Multiome | 907 | |
| chr19:1,940,240–1,942,987 | 110.3 kb | Distal (>10kb) Multiome | 901 | |
| chr19:2,034,637–2,035,967 | 16.2 kb | Distal (>10kb) Multiome | 368 | |
| chr19:2,041,744–2,042,811 | 9.0 kb | Proximal (<10kb) Multiome | 586 | |
| chr19:2,049,171–2,052,365 | 51 bp | At TSS Multiome | 977 | |
| chr19:2,060,703–2,062,560 | 10.3 kb | Distal (>10kb) Multiome | 913 | |
| chr19:2,095,898–2,097,954 | 45.4 kb | Distal (>10kb) Multiome | 945 | |
| chr19:2,150,726–2,152,200 | 100.5 kb | Distal (>10kb) Multiome | 761 | |
| chr19:2,163,561–2,164,587 | 112.6 kb | Distal (>10kb) Multiome | 750 | |
| chr19:2,235,315–2,237,253 | 185.4 kb | Distal (>10kb) Multiome | 1119 | |
| chr19:2,269,007–2,271,589 | 218.2 kb | Distal (>10kb) Multiome | 983 | |
| chr19:2,273,467–2,274,136 | 222.6 kb | Distal (>10kb) Multiome | 825 | |
| chr19:2,327,828–2,329,150 | 277.4 kb | Distal (>10kb) Multiome | 759 |
Genomic view of the MKNK2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.