This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1). [provided by RefSeq, Jun 2013]
Transcription factors with Perturb-seq knockdown data for MIB1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MIB1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MIB1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr18:21,599,940–21,601,513 | 104.2 kb | Distal (>10kb) Multiome | 1132 | |
| chr18:21,611,922–21,612,689 | 92.7 kb | Distal (>10kb) Multiome | 1050 | |
| chr18:21,703,851–21,705,209 | 206 bp | At TSS Multiome | 801 | |
| chr18:21,735,626–21,736,091 | 4.7 kb | Proximal (<10kb) | 301 | |
| chr18:21,740,314–21,741,964 | 35.8 kb | Distal (>10kb) Multiome | 803 | |
| chr18:21,754,780–21,755,646 | 50.3 kb | Distal (>10kb) Multiome | 105 | |
| chr18:21,912,423–21,913,408 | 207.9 kb | Distal (>10kb) Multiome | 73 | |
| chr18:21,933,673–21,935,412 | 229.8 kb | Distal (>10kb) Multiome | 335 | |
| chr18:21,990,759–21,991,669 | 286.3 kb | Distal (>10kb) Multiome | 354 | |
| chr18:22,020,051–22,020,863 | 315.5 kb | Distal (>10kb) Multiome | 228 | |
| chr18:22,026,039–22,027,477 | 322.0 kb | Distal (>10kb) Multiome | 321 |
Genomic view of the MIB1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.