This gene encodes a protein with similarity to a bovine microfibril-associated protein. The protein has binding specificities for both collagen and carbohydrate. It is thought to be an extracellular matrix protein which is involved in cell adhesion or intercellular interactions. The gene is located within the Smith-Magenis syndrome region. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
Transcription factors with Perturb-seq knockdown data for MFAP4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MFAP4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MFAP4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:19,237,368–19,238,388 | 149.7 kb | Distal (>10kb) Multiome | 509 | |
| chr17:19,317,172–19,317,657 | 69.8 kb | Distal (>10kb) Multiome | 467 | |
| chr17:19,362,100–19,363,673 | 24.3 kb | Distal (>10kb) Multiome | 804 | |
| chr17:19,377,561–19,379,155 | 8.9 kb | Proximal (<10kb) Multiome | 877 | |
| chr17:19,386,868–19,387,884 | 85 bp | At TSS Multiome | 305 | |
| chr17:19,433,247–19,433,662 | 46.4 kb | Distal (>10kb) Multiome | 30 | |
| chr17:19,455,896–19,456,558 | 69.0 kb | Distal (>10kb) Multiome | 43 | |
| chr17:19,457,529–19,459,378 | 70.9 kb | Distal (>10kb) Multiome | 100 | |
| chr17:19,460,682–19,461,395 | 74.0 kb | Distal (>10kb) Multiome | 93 | |
| chr17:19,507,283–19,508,789 | 120.9 kb | Distal (>10kb) Multiome | 842 | |
| chr17:19,519,787–19,520,274 | 132.8 kb | Distal (>10kb) Multiome | 130 | |
| chr17:19,533,700–19,534,412 | 146.7 kb | Distal (>10kb) Multiome | 488 | |
| chr17:19,645,969–19,646,517 | 259.0 kb | Distal (>10kb) Multiome | 116 | |
| chr17:19,647,149–19,649,280 | 260.3 kb | Distal (>10kb) Multiome | 1126 | |
| chr17:19,744,539–19,745,417 | 357.9 kb | Distal (>10kb) Multiome HiCAR | 325 |
Genomic view of the MFAP4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.