The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009]
Transcription factors with Perturb-seq knockdown data for MED12. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MED12 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MED12, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chrX:70,930,282–70,931,432 | 187.5 kb | Distal (>10kb) Multiome | 148 | |
| chrX:71,053,141–71,053,663 | 65.2 kb | Distal (>10kb) Multiome | 90 | |
| chrX:71,067,771–71,068,927 | 50.1 kb | Distal (>10kb) Multiome | 846 | |
| chrX:71,095,671–71,096,651 | 22.6 kb | Distal (>10kb) Multiome | 450 | |
| chrX:71,103,137–71,103,903 | 15.1 kb | Distal (>10kb) Multiome | 375 | |
| chrX:71,118,376–71,118,796 | at TSS | At TSS | 349 | |
| chrX:71,181,957–71,182,785 | 63.6 kb | Distal (>10kb) Multiome | 615 | |
| chrX:71,253,749–71,255,053 | 135.6 kb | Distal (>10kb) Multiome | 607 | |
| chrX:71,283,100–71,284,134 | 164.9 kb | Distal (>10kb) Multiome | 633 | |
| chrX:71,365,705–71,366,636 | 247.5 kb | Distal (>10kb) Multiome | 578 |
Genomic view of the MED12 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.