The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2018]
Transcription factors with Perturb-seq knockdown data for MCM3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MCM3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MCM3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:52,076,807–52,077,726 | 207.5 kb | Distal (>10kb) Multiome | 279 | |
| chr6:52,155,932–52,156,462 | 128.5 kb | Distal (>10kb) Multiome | 260 | |
| chr6:52,284,162–52,285,425 | 131 bp | At TSS Multiome | 958 | |
| chr6:52,286,931–52,287,092 | 2.2 kb | Proximal (<10kb) | 362 | |
| chr6:52,307,052–52,307,665 | 22.5 kb | Distal (>10kb) Multiome | 164 | |
| chr6:52,325,495–52,326,616 | 41.3 kb | Distal (>10kb) Multiome | 519 | |
| chr6:52,361,573–52,363,274 | 77.4 kb | Distal (>10kb) Multiome | 796 | |
| chr6:52,413,171–52,413,920 | 128.8 kb | Distal (>10kb) Multiome HiCAR | 46 | |
| chr6:52,419,778–52,421,057 | 135.5 kb | Distal (>10kb) Multiome HiCAR | 913 | |
| chr6:52,576,332–52,577,737 | 292.4 kb | Distal (>10kb) Multiome | 1007 | |
| chr6:52,664,088–52,665,240 | 379.6 kb | Distal (>10kb) Multiome HiCAR | 857 |
Genomic view of the MCM3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.