The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]
Transcription factors with Perturb-seq knockdown data for MAPK8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MAPK8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MAPK8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:48,274,619–48,275,163 | 31.7 kb | Distal (>10kb) Multiome | 67 | |
| chr10:48,284,741–48,285,439 | 21.6 kb | Distal (>10kb) Multiome | 48 | |
| chr10:48,306,102–48,307,484 | 84 bp | At TSS Multiome | 956 | |
| chr10:48,399,071–48,400,098 | 92.9 kb | Distal (>10kb) Multiome | 129 | |
| chr10:48,444,253–48,445,663 | 137.9 kb | Distal (>10kb) Multiome | 245 | |
| chr10:48,469,724–48,470,675 | 163.6 kb | Distal (>10kb) Multiome | 521 | |
| chr10:48,523,248–48,524,705 | 217.7 kb | Distal (>10kb) Multiome | 466 | |
| chr10:48,537,011–48,537,486 | 230.5 kb | Distal (>10kb) Multiome | 35 | |
| chr10:48,586,969–48,587,710 | 280.6 kb | Distal (>10kb) Multiome | 50 |
Genomic view of the MAPK8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.