MAFG

MAF bZIP transcription factor G | MGC13090, MGC20149

Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM, Mar 2010]

Developmental clusters: GC6

Biological processes 21 terms

DNA binding (GO:0003677)DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)identical protein binding (GO:0042802)negative regulation of transcription by RNA polymerase II (GO:0000122)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)positive regulation of gene expression (GO:0010628)positive regulation of transcription by RNA polymerase II (GO:0045944)protein binding (GO:0005515)regulation of DNA-templated transcription (GO:0006355)regulation of epidermal cell differentiation (GO:0045604)regulation of transcription by RNA polymerase II (GO:0006357)regulation of transcription by RNA polymerase II (GO:0006357)sequence-specific double-stranded DNA binding (GO:1990837)

Expression (TPM)

TFs regulating MAFG 0 TFs

Transcription factors with Perturb-seq knockdown data for MAFG. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MAFG upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to MAFG

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MAFG, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr17:81,636,404–81,637,301	290.7 kb	Distal (>10kb) Multiome	946
chr17:81,648,048–81,648,550	279.5 kb	Distal (>10kb) Multiome	88
chr17:81,652,970–81,653,572	274.6 kb	Distal (>10kb) Multiome	188
chr17:81,666,251–81,667,166	261.1 kb	Distal (>10kb) Multiome	901
chr17:81,683,344–81,684,556	243.8 kb	Distal (>10kb) Multiome	926
chr17:81,702,879–81,703,868	224.4 kb	Distal (>10kb) Multiome	981
chr17:81,711,721–81,712,812	215.5 kb	Distal (>10kb) Multiome	1056
chr17:81,832,473–81,833,976	94.5 kb	Distal (>10kb) Multiome	1015
chr17:81,859,762–81,861,595	66.9 kb	Distal (>10kb) Multiome	1134
chr17:81,868,421–81,868,978	59.2 kb	Distal (>10kb) Multiome	577
chr17:81,869,000–81,872,289	56.5 kb	Distal (>10kb) Multiome	1136
chr17:81,891,143–81,892,257	36.0 kb	Distal (>10kb) Multiome	969
chr17:81,910,443–81,911,980	16.2 kb	Distal (>10kb) Multiome	806
chr17:81,917,586–81,918,522	9.6 kb	Proximal (<10kb) Multiome	619
chr17:81,923,285–81,923,863	4.2 kb	Proximal (<10kb) Multiome	399
chr17:81,924,942–81,925,146	2.6 kb	Proximal (<10kb)	273
chr17:81,926,141–81,929,133	155 bp	At TSS Multiome	1034
chr17:81,936,855–81,937,682	9.6 kb	Proximal (<10kb) Multiome	854
chr17:81,959,299–81,962,169	32.0 kb	Distal (>10kb) Multiome	805
chr17:81,966,804–81,968,359	40.3 kb	Distal (>10kb) Multiome	908
chr17:81,976,595–81,978,312	49.9 kb	Distal (>10kb) Multiome	1001
chr17:82,019,856–82,020,391	92.4 kb	Distal (>10kb) Multiome	436
chr17:82,022,355–82,024,111	95.3 kb	Distal (>10kb) Multiome	898
chr17:82,029,976–82,032,662	104.2 kb	Distal (>10kb) Multiome	695
chr17:82,036,902–82,037,961	109.9 kb	Distal (>10kb) Multiome	892
chr17:82,051,387–82,052,184	124.0 kb	Distal (>10kb) Multiome	479
chr17:82,065,558–82,066,150	138.1 kb	Distal (>10kb) Multiome	967
chr17:82,096,478–82,100,203	170.8 kb	Distal (>10kb) Multiome	1085
chr17:82,103,334–82,104,356	175.8 kb	Distal (>10kb) Multiome	672
chr17:82,212,405–82,213,592	285.2 kb	Distal (>10kb) Multiome	911

Genome Browser

Genomic view of the MAFG locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.