LIMA1
LIM domain and actin binding 1 | EPLIN

This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011]

Member of: DE-4 DE-4.25 Developmental clusters: GC3
Biological processes 45 terms
actin cytoskeleton (GO:0015629)actin cytoskeleton (GO:0015629)actin cytoskeleton (GO:0015629)actin filament (GO:0005884)actin filament binding (GO:0051015)actin filament binding (GO:0051015)actin filament binding (GO:0051015)actin filament bundle assembly (GO:0051017)actin filament bundle assembly (GO:0051017)actin monomer binding (GO:0003785)brush border (GO:0005903)brush border membrane (GO:0031526)brush border membrane (GO:0031526)cadherin binding (GO:0045296)cell migration (GO:0016477)cell migration (GO:0016477)cholesterol homeostasis (GO:0042632)cholesterol homeostasis (GO:0042632)cleavage furrow (GO:0032154)cytoplasm (GO:0005737)cytoskeleton (GO:0005856)cytosol (GO:0005829)focal adhesion (GO:0005925)focal adhesion (GO:0005925)focal adhesion (GO:0005925)intestinal cholesterol absorption (GO:0030299)intestinal cholesterol absorption (GO:0030299)lamellipodium (GO:0030027)lamellipodium (GO:0030027)microtubule stabilizing activity (GO:0140778)negative regulation of actin filament depolymerization (GO:0030835)negative regulation of cell migration (GO:0030336)negative regulation of cilium assembly (GO:1902018)plasma membrane (GO:0005886)plasma membrane (GO:0005886)positive regulation of actin filament bundle assembly (GO:0032233)protein binding (GO:0005515)regulation of lamellipodium organization (GO:1902743)ruffle (GO:0001726)ruffle (GO:0001726)ruffle (GO:0001726)ruffle organization (GO:0031529)ruffle organization (GO:0031529)stress fiber (GO:0001725)stress fiber (GO:0001725)
Expression (TPM)
LIMA1 — as a Regulated Gene

TFs regulating LIMA1 0 TFs

Transcription factors with Perturb-seq knockdown data for LIMA1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = LIMA1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to LIMA1

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of LIMA1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr12:49,945,408–49,945,886 337.8 kb Distal (>10kb) Multiome 520
chr12:49,961,271–49,962,190 321.8 kb Distal (>10kb) Multiome 164
chr12:49,966,854–49,967,924 316.1 kb Distal (>10kb) Multiome 698
chr12:50,024,956–50,025,990 257.9 kb Distal (>10kb) Multiome 829
chr12:50,050,458–50,051,443 232.6 kb Distal (>10kb) Multiome 412
chr12:50,057,263–50,058,231 225.8 kb Distal (>10kb) Multiome 437
chr12:50,084,798–50,085,859 198.2 kb Distal (>10kb) Multiome 715
chr12:50,110,765–50,112,744 171.5 kb Distal (>10kb) Multiome 967
chr12:50,166,692–50,167,931 116.0 kb Distal (>10kb) Multiome 812
chr12:50,222,571–50,222,760 at TSS At TSS 274
chr12:50,224,425–50,224,593 1.7 kb Proximal (<10kb) 246
chr12:50,232,842–50,233,291 50.4 kb Distal (>10kb) Multiome HiCAR 105
chr12:50,283,141–50,284,081 56 bp At TSS Multiome HiCAR 750
chr12:50,400,518–50,401,658 117.4 kb Distal (>10kb) Multiome 948
chr12:50,504,583–50,505,803 221.5 kb Distal (>10kb) Multiome 806

Genome Browser

Genomic view of the LIMA1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr12:49,935,408 – 50,515,803
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq