Transcription factors with Perturb-seq knockdown data for LHX8-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = LHX8-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of LHX8-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:75,124,347–75,125,401 | 7.2 kb | Proximal (<10kb) | 143 | |
| chr1:75,127,900–75,128,460 | 4.1 kb | Proximal (<10kb) | 28 | |
| chr1:75,129,490–75,131,873 | 702 bp | At TSS | 209 | |
| chr1:75,132,114–75,132,725 | at TSS | At TSS | 52 | |
| chr1:75,133,083–75,133,477 | 507 bp | At TSS | 40 | |
| chr1:75,133,645–75,137,727 | 1.1 kb | Proximal (<10kb) | 495 |
Genomic view of the LHX8-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.