Transcription factors with Perturb-seq knockdown data for LENG8-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = LENG8-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of LENG8-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:54,159,030–54,160,865 | 289.2 kb | Distal (>10kb) Multiome | 793 | |
| chr19:54,161,897–54,162,738 | 286.6 kb | Distal (>10kb) Multiome | 233 | |
| chr19:54,164,508–54,165,013 | 284.2 kb | Distal (>10kb) Multiome | 183 | |
| chr19:54,172,536–54,173,532 | 275.9 kb | Distal (>10kb) Multiome | 397 | |
| chr19:54,188,192–54,192,172 | 259.1 kb | Distal (>10kb) Multiome | 979 | |
| chr19:54,200,383–54,201,351 | 248.2 kb | Distal (>10kb) Multiome | 891 | |
| chr19:54,208,780–54,209,556 | 239.6 kb | Distal (>10kb) Multiome | 358 | |
| chr19:54,231,547–54,232,136 | 217.2 kb | Distal (>10kb) Multiome | 471 | |
| chr19:54,420,287–54,421,161 | 28.2 kb | Distal (>10kb) Multiome | 334 | |
| chr19:54,430,381–54,431,285 | 18.0 kb | Distal (>10kb) Multiome | 240 | |
| chr19:54,439,510–54,439,707 | 9.3 kb | Proximal (<10kb) | 142 | |
| chr19:54,448,346–54,449,853 | 194 bp | At TSS Multiome | 959 | |
| chr19:54,462,469–54,465,494 | 14.9 kb | Distal (>10kb) Multiome | 1004 | |
| chr19:55,117,134–55,119,109 | 668.7 kb | Distal (>10kb) Multiome HiCAR | 881 |
Genomic view of the LENG8-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.