This gene encodes a member of the kinesin-3 superfamily of microtubule motor proteins. These proteins are involved in numerous processes including vesicle transport, chromosome segregation, mitotic spindle formation, and cytokinesis. In human HeLa-S3 and 293T cells, this protein is localized to the cytoplasm during interphase, to the spindle poles and spindle microtubules during mitosis, and to the midbody during cytokinesis. An internal motor domain displays microtubule-dependent ATPase activity, consistent with its function as a microtubule motor protein. Knockdown of this gene results in failed cytokinesis with endoreplication, which results in multinucleated cells. This gene has been identified as a likely oncogene in breast, lung and ovarian cancers, as well as retinoblastomas and gliomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]
Transcription factors with Perturb-seq knockdown data for KIF14. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = KIF14 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of KIF14, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:200,364,189–200,365,401 | 255.8 kb | Distal (>10kb) Multiome | 292 | |
| chr1:200,368,023–200,369,613 | 252.1 kb | Distal (>10kb) Multiome | 213 | |
| chr1:200,409,102–200,410,859 | 210.6 kb | Distal (>10kb) Multiome | 818 | |
| chr1:200,411,526–200,412,290 | 209.0 kb | Distal (>10kb) Multiome | 202 | |
| chr1:200,620,328–200,621,096 | 60 bp | At TSS Multiome | 739 | |
| chr1:200,669,293–200,670,631 | 49.2 kb | Distal (>10kb) Multiome | 1078 | |
| chr1:200,738,299–200,740,095 | 118.2 kb | Distal (>10kb) Multiome | 831 | |
| chr1:200,890,766–200,892,246 | 270.6 kb | Distal (>10kb) Multiome | 761 | |
| chr1:200,915,808–200,916,700 | 295.6 kb | Distal (>10kb) Multiome | 219 |
Genomic view of the KIF14 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.