The protein encoded by this gene is a cytoplasmic membrane-associated protein that indirectly coordinates endocytic membrane traffic with the actin assembly machinery. In addition, the encoded protein may regulate the formation of clathrin-coated vesicles and could be involved in synaptic vesicle recycling. This protein has been shown to interact with dynamin, CDC42, SNAP23, SNAP25, SPIN90, EPS15, EPN1, EPN2, and STN2. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been characterized so far. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for ITSN1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ITSN1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ITSN1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr21:33,402,567–33,402,985 | 239.6 kb | Distal (>10kb) Multiome | 218 | |
| chr21:33,403,116–33,404,351 | 239.1 kb | Distal (>10kb) Multiome | 570 | |
| chr21:33,478,861–33,480,615 | 162.4 kb | Distal (>10kb) Multiome | 839 | |
| chr21:33,491,033–33,492,145 | 150.9 kb | Distal (>10kb) Multiome | 740 | |
| chr21:33,541,871–33,543,585 | 100.5 kb | Distal (>10kb) Multiome | 996 | |
| chr21:33,587,947–33,589,340 | 53.7 kb | Distal (>10kb) Multiome | 866 | |
| chr21:33,641,108–33,643,411 | 390 bp | At TSS Multiome | 1018 | |
| chr21:33,651,077–33,651,511 | 8.6 kb | Proximal (<10kb) | 61 | |
| chr21:33,652,026–33,652,412 | 9.5 kb | Proximal (<10kb) | 55 | |
| chr21:33,894,789–33,895,286 | 252.5 kb | Distal (>10kb) Multiome HiCAR | 371 | |
| chr21:33,915,370–33,916,223 | 273.4 kb | Distal (>10kb) Multiome | 730 |
Genomic view of the ITSN1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.