Transcription factors with Perturb-seq knockdown data for ITGB5-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ITGB5-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ITGB5-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:124,545,526–124,546,488 | 235.1 kb | Distal (>10kb) Multiome | 30 | |
| chr3:124,584,341–124,585,325 | 196.4 kb | Distal (>10kb) Multiome | 571 | |
| chr3:124,730,263–124,730,718 | 50.7 kb | Distal (>10kb) Multiome | 844 | |
| chr3:124,757,599–124,758,508 | 23.2 kb | Distal (>10kb) Multiome | 59 | |
| chr3:124,780,800–124,781,289 | at TSS | At TSS | 145 | |
| chr3:124,886,097–124,888,040 | 106.4 kb | Distal (>10kb) Multiome HiCAR | 804 | |
| chr3:124,888,393–124,889,745 | 108.1 kb | Distal (>10kb) Multiome HiCAR | 135 | |
| chr3:124,910,445–124,911,085 | 129.6 kb | Distal (>10kb) Multiome | 133 | |
| chr3:125,055,367–125,056,705 | 275.0 kb | Distal (>10kb) Multiome | 524 |
Genomic view of the ITGB5-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.