This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
Transcription factors with Perturb-seq knockdown data for IST1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = IST1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of IST1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:71,625,835–71,627,289 | 269.3 kb | Distal (>10kb) Multiome | 594 | |
| chr16:71,722,829–71,724,442 | 172.4 kb | Distal (>10kb) Multiome | 894 | |
| chr16:71,808,251–71,809,603 | 86.4 kb | Distal (>10kb) Multiome | 1032 | |
| chr16:71,845,428–71,846,392 | 49.6 kb | Distal (>10kb) Multiome | 923 | |
| chr16:71,883,009–71,884,742 | 11.3 kb | Distal (>10kb) Multiome | 791 | |
| chr16:71,890,687–71,891,083 | 4.4 kb | Proximal (<10kb) | 114 | |
| chr16:71,894,288–71,896,022 | 98 bp | At TSS Multiome | 1139 | |
| chr16:71,981,752–71,982,390 | 86.6 kb | Distal (>10kb) Multiome | 263 | |
| chr16:72,008,426–72,009,172 | 113.2 kb | Distal (>10kb) Multiome | 815 | |
| chr16:72,013,585–72,014,228 | 118.6 kb | Distal (>10kb) Multiome | 263 | |
| chr16:72,093,288–72,094,355 | 198.2 kb | Distal (>10kb) Multiome | 860 | |
| chr16:72,111,833–72,112,319 | 216.6 kb | Distal (>10kb) Multiome | 60 |
Genomic view of the IST1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.