The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14. [provided by RefSeq, Dec 2014]
Transcription factors with Perturb-seq knockdown data for ILF2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ILF2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ILF2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:153,494,904–153,495,543 | 175.9 kb | Distal (>10kb) Multiome | 290 | |
| chr1:153,532,833–153,533,594 | 137.8 kb | Distal (>10kb) Multiome | 493 | |
| chr1:153,538,198–153,538,657 | 132.5 kb | Distal (>10kb) Multiome | 338 | |
| chr1:153,544,844–153,545,543 | 125.8 kb | Distal (>10kb) Multiome | 575 | |
| chr1:153,568,278–153,569,058 | 102.3 kb | Distal (>10kb) Multiome | 641 | |
| chr1:153,612,574–153,613,563 | 57.9 kb | Distal (>10kb) Multiome | 388 | |
| chr1:153,626,535–153,627,974 | 43.4 kb | Distal (>10kb) Multiome | 697 | |
| chr1:153,633,575–153,634,639 | 36.8 kb | Distal (>10kb) Multiome | 998 | |
| chr1:153,658,331–153,659,230 | 12.2 kb | Distal (>10kb) Multiome | 746 | |
| chr1:153,670,709–153,671,992 | 175 bp | At TSS Multiome | 884 | |
| chr1:153,677,768–153,677,972 | 6.8 kb | Proximal (<10kb) | 282 | |
| chr1:153,679,083–153,679,923 | 8.1 kb | Proximal (<10kb) | 349 | |
| chr1:153,679,997–153,680,373 | 9.0 kb | Proximal (<10kb) | 61 | |
| chr1:153,697,776–153,698,654 | 27.4 kb | Distal (>10kb) Multiome | 313 | |
| chr1:153,727,492–153,728,827 | 56.9 kb | Distal (>10kb) Multiome | 702 | |
| chr1:153,736,782–153,737,426 | 66.1 kb | Distal (>10kb) Multiome | 10 | |
| chr1:153,745,487–153,745,959 | 74.7 kb | Distal (>10kb) Multiome HiCAR | 55 | |
| chr1:153,774,962–153,776,698 | 104.2 kb | Distal (>10kb) Multiome HiCAR | 671 | |
| chr1:153,783,341–153,784,300 | 112.7 kb | Distal (>10kb) Multiome | 794 | |
| chr1:153,922,213–153,923,779 | 252.1 kb | Distal (>10kb) Multiome | 856 | |
| chr1:153,945,717–153,947,118 | 275.7 kb | Distal (>10kb) Multiome | 866 | |
| chr1:153,957,828–153,959,103 | 287.7 kb | Distal (>10kb) Multiome | 912 | |
| chr1:153,963,107–153,964,407 | 292.7 kb | Distal (>10kb) Multiome | 798 | |
| chr1:153,967,147–153,968,324 | 296.7 kb | Distal (>10kb) Multiome | 986 |
Genomic view of the ILF2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.