The protein encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biological activities. This enzyme is a type II integral membrane protein and is responsible for 6-O-sulfation of heparan sulfate. This enzyme does not share significant sequence similarity with other known sulfotransferases. A pseudogene located on chromosome 1 has been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for HS6ST1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HS6ST1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HS6ST1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:128,026,821–128,028,727 | 291.5 kb | Distal (>10kb) Multiome | 872 | |
| chr2:128,090,830–128,091,736 | 227.7 kb | Distal (>10kb) Multiome | 987 | |
| chr2:128,195,741–128,196,874 | 122.5 kb | Distal (>10kb) Multiome HiCAR | 365 | |
| chr2:128,232,712–128,234,252 | 85.3 kb | Distal (>10kb) Multiome HiCAR | 834 | |
| chr2:128,234,465–128,235,115 | 84.0 kb | Distal (>10kb) Multiome HiCAR | 66 | |
| chr2:128,260,688–128,261,545 | 57.9 kb | Distal (>10kb) Multiome HiCAR | 352 | |
| chr2:128,309,377–128,309,845 | 9.0 kb | Proximal (<10kb) | 720 | |
| chr2:128,317,236–128,318,078 | 1.4 kb | Proximal (<10kb) Multiome | 437 | |
| chr2:128,318,609–128,319,523 | 107 bp | At TSS Multiome | 550 | |
| chr2:128,319,645–128,319,965 | 777 bp | At TSS | 78 | |
| chr2:128,321,402–128,322,797 | 3.1 kb | Proximal (<10kb) Multiome | 727 | |
| chr2:128,378,778–128,379,757 | 60.4 kb | Distal (>10kb) Multiome | 353 | |
| chr2:128,413,366–128,414,666 | 95.1 kb | Distal (>10kb) Multiome HiCAR | 232 | |
| chr2:128,424,487–128,425,506 | 106.1 kb | Distal (>10kb) Multiome | 165 | |
| chr2:128,532,910–128,533,444 | 214.4 kb | Distal (>10kb) Multiome | 75 | |
| chr2:128,638,793–128,639,389 | 320.1 kb | Distal (>10kb) Multiome HiCAR | 178 | |
| chr2:128,644,705–128,645,527 | 326.2 kb | Distal (>10kb) Multiome HiCAR | 167 | |
| chr2:128,736,466–128,737,432 | 418.1 kb | Distal (>10kb) Multiome HiCAR | 326 | |
| chr2:129,827,287–129,827,983 | 1508.8 kb | Distal (>10kb) Multiome HiCAR | 180 | |
| chr2:129,877,126–129,878,386 | 1558.8 kb | Distal (>10kb) Multiome HiCAR | 356 | |
| chr2:129,923,163–129,924,117 | 1604.7 kb | Distal (>10kb) Multiome HiCAR | 183 |
Genomic view of the HS6ST1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.