This gene encodes the enzyme heparan sulfate D-glucosaminyl 3-O-sulfotransferase 4. This enzyme generates 3-O-sulfated glucosaminyl residues in heparan sulfate. Cell surface heparan sulfate is used as a receptor by herpes simplex virus type 1 (HSV-1), and expression of this gene is thought to play a role in HSV-1 pathogenesis. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for HS3ST4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HS3ST4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HS3ST4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:25,691,565–25,692,134 | 3 bp | At TSS Multiome | 144 | |
| chr16:25,692,205–25,693,123 | 247 bp | At TSS | 117 | |
| chr16:25,855,688–25,856,393 | 164.1 kb | Distal (>10kb) Multiome | 77 | |
| chr16:25,950,594–25,951,289 | 259.0 kb | Distal (>10kb) Multiome | 265 | |
| chr16:26,606,278–26,607,172 | 914.7 kb | Distal (>10kb) Multiome HiCAR | 110 | |
| chr16:27,109,686–27,110,564 | 1418.0 kb | Distal (>10kb) Multiome HiCAR | 416 |
Genomic view of the HS3ST4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.