Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016]
Transcription factors with Perturb-seq knockdown data for HK1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HK1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HK1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:69,123,352–69,125,173 | 194.8 kb | Distal (>10kb) Multiome | 918 | |
| chr10:69,179,817–69,180,725 | 138.7 kb | Distal (>10kb) Multiome | 900 | |
| chr10:69,317,873–69,319,610 | 172 bp | At TSS Multiome | 675 | |
| chr10:69,329,639–69,330,213 | 11.1 kb | Distal (>10kb) Multiome | 155 | |
| chr10:69,375,626–69,376,241 | 57.1 kb | Distal (>10kb) Multiome | 178 | |
| chr10:69,392,338–69,392,832 | 73.8 kb | Distal (>10kb) Multiome | 454 | |
| chr10:69,408,469–69,409,338 | 90.0 kb | Distal (>10kb) Multiome | 705 | |
| chr10:69,449,843–69,450,266 | 131.1 kb | Distal (>10kb) Multiome | 301 | |
| chr10:69,450,797–69,452,284 | 132.6 kb | Distal (>10kb) Multiome | 508 | |
| chr10:69,568,733–69,569,392 | 250.2 kb | Distal (>10kb) Multiome | 99 | |
| chr10:69,573,228–69,573,810 | 254.7 kb | Distal (>10kb) Multiome | 284 |
Genomic view of the HK1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.