The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
Transcription factors with Perturb-seq knockdown data for HIRIP3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HIRIP3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HIRIP3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:29,708,839–29,709,431 | 287.0 kb | Distal (>10kb) Multiome | 96 | |
| chr16:29,709,743–29,710,091 | 286.1 kb | Distal (>10kb) Multiome | 21 | |
| chr16:29,784,957–29,785,426 | 210.8 kb | Distal (>10kb) Multiome | 452 | |
| chr16:29,790,073–29,791,192 | 205.5 kb | Distal (>10kb) Multiome | 1003 | |
| chr16:29,804,592–29,808,172 | 190.3 kb | Distal (>10kb) Multiome | 1275 | |
| chr16:29,810,746–29,812,649 | 183.7 kb | Distal (>10kb) Multiome | 826 | |
| chr16:29,815,828–29,817,186 | 179.9 kb | Distal (>10kb) Multiome | 1006 | |
| chr16:29,862,597–29,863,901 | 132.6 kb | Distal (>10kb) Multiome | 953 | |
| chr16:29,876,281–29,878,282 | 118.4 kb | Distal (>10kb) Multiome | 319 | |
| chr16:29,899,174–29,900,833 | 96.6 kb | Distal (>10kb) Multiome | 1019 | |
| chr16:29,925,692–29,926,784 | 69.8 kb | Distal (>10kb) Multiome | 912 | |
| chr16:29,961,477–29,962,530 | 34.1 kb | Distal (>10kb) Multiome | 848 | |
| chr16:29,973,238–29,974,906 | 22.4 kb | Distal (>10kb) Multiome | 935 | |
| chr16:29,994,888–29,996,728 | 74 bp | At TSS Multiome | 1080 | |
| chr16:30,021,215–30,021,860 | 25.5 kb | Distal (>10kb) Multiome | 764 | |
| chr16:30,052,803–30,053,364 | 57.0 kb | Distal (>10kb) Multiome | 989 | |
| chr16:30,063,722–30,066,296 | 68.0 kb | Distal (>10kb) Multiome | 1209 | |
| chr16:30,075,490–30,076,439 | 79.9 kb | Distal (>10kb) Multiome | 828 | |
| chr16:30,090,881–30,092,910 | 96.8 kb | Distal (>10kb) Multiome | 1101 | |
| chr16:30,095,557–30,096,839 | 100.3 kb | Distal (>10kb) Multiome | 824 | |
| chr16:30,122,728–30,123,578 | 127.2 kb | Distal (>10kb) Multiome | 718 | |
| chr16:30,182,759–30,183,865 | 187.4 kb | Distal (>10kb) Multiome | 547 |
Genomic view of the HIRIP3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.