HEY2
hes related family bHLH transcription factor with YRPW motif 2 | HERP1, HESR2, bHLHb32

This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The encoded protein forms homo- or hetero-dimers that localize to the nucleus and interact with a histone deacetylase complex to repress transcription. Expression of this gene is induced by the Notch signal transduction pathway. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Member of: DE-7 DE-7.1 Developmental clusters: GC4
Biological processes 110 terms
DNA binding (GO:0003677)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription repressor activity, RNA polymerase II-specific (GO:0001227)DNA-binding transcription repressor activity, RNA polymerase II-specific (GO:0001227)DNA-binding transcription repressor activity, RNA polymerase II-specific (GO:0001227)Notch signaling pathway (GO:0007219)Notch signaling pathway (GO:0007219)Notch signaling pathway (GO:0007219)Notch signaling pathway (GO:0007219)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II-specific DNA-binding transcription factor binding (GO:0061629)RNA polymerase II-specific DNA-binding transcription factor binding (GO:0061629)anatomical structure morphogenesis (GO:0009653)anterior/posterior pattern specification (GO:0009952)aortic valve morphogenesis (GO:0003180)aortic valve morphogenesis (GO:0003180)aortic valve morphogenesis (GO:0003180)arterial endothelial cell differentiation (GO:0060842)arterial endothelial cell differentiation (GO:0060842)ascending aorta morphogenesis (GO:0035910)ascending aorta morphogenesis (GO:0035910)atrial septum morphogenesis (GO:0060413)atrial septum morphogenesis (GO:0060413)cardiac conduction system development (GO:0003161)cardiac epithelial to mesenchymal transition (GO:0060317)cardiac epithelial to mesenchymal transition (GO:0060317)cardiac left ventricle morphogenesis (GO:0003214)cardiac left ventricle morphogenesis (GO:0003214)cardiac right ventricle morphogenesis (GO:0003215)cardiac right ventricle morphogenesis (GO:0003215)cardiac septum morphogenesis (GO:0060411)cardiac septum morphogenesis (GO:0060411)cardiac ventricle morphogenesis (GO:0003208)cardiac ventricle morphogenesis (GO:0003208)chromatin (GO:0000785)circulatory system development (GO:0072359)circulatory system development (GO:0072359)cis-regulatory region sequence-specific DNA binding (GO:0000987)cis-regulatory region sequence-specific DNA binding (GO:0000987)cytoplasm (GO:0005737)cytoplasm (GO:0005737)dorsal aorta morphogenesis (GO:0035912)dorsal aorta morphogenesis (GO:0035912)epithelial to mesenchymal transition involved in endocardial cushion formation (GO:0003198)heart development (GO:0007507)heart trabecula formation (GO:0060347)histone deacetylase binding (GO:0042826)identical protein binding (GO:0042802)labyrinthine layer blood vessel development (GO:0060716)labyrinthine layer blood vessel development (GO:0060716)mesenchymal cell development (GO:0014031)mesenchymal cell development (GO:0014031)muscular septum morphogenesis (GO:0003150)muscular septum morphogenesis (GO:0003150)negative regulation of DNA-templated transcription (GO:0045892)negative regulation of DNA-templated transcription (GO:0045892)negative regulation of Notch signaling pathway (GO:0045746)negative regulation of Notch signaling pathway (GO:0045746)negative regulation of biomineral tissue development (GO:0070168)negative regulation of biomineral tissue development (GO:0070168)negative regulation of cardiac vascular smooth muscle cell differentiation (GO:2000723)negative regulation of cardiac vascular smooth muscle cell differentiation (GO:2000723)negative regulation of gene expression (GO:0010629)negative regulation of gene expression (GO:0010629)negative regulation of smooth muscle cell differentiation (GO:0051151)negative regulation of smooth muscle cell differentiation (GO:0051151)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of transcription initiation by RNA polymerase II (GO:0060633)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)outflow tract morphogenesis (GO:0003151)outflow tract morphogenesis (GO:0003151)positive regulation of transcription by RNA polymerase II (GO:0045944)positive regulation of transcription by RNA polymerase II (GO:0045944)protein binding (GO:0005515)protein dimerization activity (GO:0046983)pulmonary artery morphogenesis (GO:0061156)pulmonary artery morphogenesis (GO:0061156)pulmonary valve morphogenesis (GO:0003184)pulmonary valve morphogenesis (GO:0003184)regulation of DNA-templated transcription (GO:0006355)regulation of neurogenesis (GO:0050767)regulation of vasculogenesis (GO:2001212)regulation of vasculogenesis (GO:2001212)sequence-specific DNA binding (GO:0043565)sequence-specific DNA binding (GO:0043565)sequence-specific double-stranded DNA binding (GO:1990837)smooth muscle cell differentiation (GO:0051145)transcription repressor complex (GO:0017053)tricuspid valve morphogenesis (GO:0003186)tricuspid valve morphogenesis (GO:0003186)umbilical cord morphogenesis (GO:0036304)umbilical cord morphogenesis (GO:0036304)vascular associated smooth muscle cell development (GO:0097084)vasculogenesis (GO:0001570)ventricular cardiac muscle cell development (GO:0055015)ventricular cardiac muscle cell development (GO:0055015)ventricular septum morphogenesis (GO:0060412)ventricular septum morphogenesis (GO:0060412)
Expression (TPM)
HEY2 — as a Regulated Gene

TFs regulating HEY2 0 TFs

Transcription factors with Perturb-seq knockdown data for HEY2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HEY2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to HEY2

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HEY2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr6:125,528,427–125,529,436 220.8 kb Distal (>10kb) Multiome 266
chr6:125,700,629–125,701,546 48.5 kb Distal (>10kb) Multiome HiCAR 153
chr6:125,745,345–125,746,068 3.9 kb Proximal (<10kb) Multiome 210
chr6:125,746,584–125,747,315 2.3 kb Proximal (<10kb) 391
chr6:125,747,542–125,750,482 75 bp At TSS Multiome 891
chr6:125,759,030–125,759,610 9.4 kb Proximal (<10kb) 235
chr6:125,780,157–125,781,423 30.8 kb Distal (>10kb) Multiome 1022
chr6:125,789,677–125,791,851 41.2 kb Distal (>10kb) Multiome 1017
chr6:125,867,468–125,867,998 118.2 kb Distal (>10kb) Multiome 264
chr6:125,952,889–125,953,903 203.8 kb Distal (>10kb) Multiome 343
chr6:125,956,540–125,957,527 207.3 kb Distal (>10kb) Multiome 1085
chr6:125,986,066–125,987,257 236.9 kb Distal (>10kb) Multiome 925

Genome Browser

Genomic view of the HEY2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr6:125,518,427 – 125,997,257
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq
Huangfu Lab · Publications · Resources · Contact