This gene encodes the beta subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the beta subunit catalyzing the 3-ketoacyl-CoA thiolase activity. The encoded protein can also bind RNA and decreases the stability of some mRNAs. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. Mutations in this gene result in trifunctional protein deficiency. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013]
Transcription factors with Perturb-seq knockdown data for HADHB. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HADHB upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HADHB, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:25,981,827–25,983,286 | 262.3 kb | Distal (>10kb) Multiome | 664 | |
| chr2:26,011,658–26,012,446 | 232.8 kb | Distal (>10kb) Multiome | 485 | |
| chr2:26,033,136–26,034,956 | 211.0 kb | Distal (>10kb) Multiome | 1165 | |
| chr2:26,172,352–26,174,365 | 71.9 kb | Distal (>10kb) Multiome | 420 | |
| chr2:26,178,689–26,179,345 | 65.8 kb | Distal (>10kb) Multiome | 245 | |
| chr2:26,179,469–26,180,030 | 65.2 kb | Distal (>10kb) Multiome | 122 | |
| chr2:26,184,159–26,185,510 | 60.0 kb | Distal (>10kb) Multiome | 494 | |
| chr2:26,202,471–26,204,141 | 41.1 kb | Distal (>10kb) Multiome | 38 | |
| chr2:26,244,319–26,245,633 | 117 bp | At TSS Multiome | 928 | |
| chr2:26,345,352–26,346,859 | 101.1 kb | Distal (>10kb) Multiome | 1002 | |
| chr2:26,401,746–26,402,358 | 157.0 kb | Distal (>10kb) Multiome | 231 | |
| chr2:26,503,215–26,503,885 | 258.6 kb | Distal (>10kb) Multiome | 522 |
Genomic view of the HADHB locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.