This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for HADHA. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = HADHA upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of HADHA, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:25,981,827–25,983,286 | 262.0 kb | Distal (>10kb) Multiome | 664 | |
| chr2:26,011,658–26,012,446 | 232.5 kb | Distal (>10kb) Multiome | 485 | |
| chr2:26,033,136–26,034,956 | 210.7 kb | Distal (>10kb) Multiome | 1165 | |
| chr2:26,172,352–26,174,365 | 71.6 kb | Distal (>10kb) Multiome | 420 | |
| chr2:26,178,689–26,179,345 | 65.5 kb | Distal (>10kb) Multiome | 245 | |
| chr2:26,179,469–26,180,030 | 64.9 kb | Distal (>10kb) Multiome | 122 | |
| chr2:26,184,159–26,185,510 | 59.7 kb | Distal (>10kb) Multiome | 494 | |
| chr2:26,202,471–26,204,141 | 40.8 kb | Distal (>10kb) Multiome | 38 | |
| chr2:26,244,319–26,245,633 | 176 bp | At TSS Multiome | 928 | |
| chr2:26,345,352–26,346,859 | 101.4 kb | Distal (>10kb) Multiome | 1002 | |
| chr2:26,401,746–26,402,358 | 157.3 kb | Distal (>10kb) Multiome | 231 | |
| chr2:26,503,215–26,503,885 | 258.9 kb | Distal (>10kb) Multiome | 522 |
Genomic view of the HADHA locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.