Transcription factors with Perturb-seq knockdown data for GUSBP2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GUSBP2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GUSBP2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:26,595,619–26,596,282 | 360.7 kb | Distal (>10kb) Multiome | 798 | |
| chr6:26,596,497–26,597,408 | 359.6 kb | Distal (>10kb) Multiome | 940 | |
| chr6:26,659,338–26,660,351 | 296.8 kb | Distal (>10kb) Multiome | 748 | |
| chr6:26,956,244–26,957,132 | 199 bp | At TSS Multiome | 399 | |
| chr6:27,019,699–27,020,456 | 63.6 kb | Distal (>10kb) Multiome | 707 | |
| chr6:27,047,461–27,047,994 | 91.3 kb | Distal (>10kb) Multiome | 28 | |
| chr6:27,049,960–27,050,724 | 93.7 kb | Distal (>10kb) Multiome | 99 | |
| chr6:27,091,728–27,092,257 | 135.4 kb | Distal (>10kb) Multiome | 610 | |
| chr6:27,131,467–27,132,107 | 175.1 kb | Distal (>10kb) Multiome | 580 | |
| chr6:27,132,200–27,134,276 | 176.4 kb | Distal (>10kb) Multiome | 975 | |
| chr6:27,139,091–27,140,128 | 182.9 kb | Distal (>10kb) Multiome | 662 | |
| chr6:27,145,480–27,148,111 | 190.5 kb | Distal (>10kb) Multiome | 951 | |
| chr6:27,177,493–27,178,620 | 221.5 kb | Distal (>10kb) Multiome | 685 | |
| chr6:27,188,443–27,189,128 | 232.2 kb | Distal (>10kb) Multiome | 398 | |
| chr6:27,214,739–27,215,257 | 258.5 kb | Distal (>10kb) Multiome | 338 | |
| chr6:27,230,181–27,230,668 | 273.9 kb | Distal (>10kb) Multiome | 624 | |
| chr6:27,250,707–27,252,203 | 295.2 kb | Distal (>10kb) Multiome | 736 |
Genomic view of the GUSBP2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.