GRB2
growth factor receptor bound protein 2 | NCKAP2

The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Member of: DE-10 DE-10.4
Biological processes 66 terms
B cell receptor signaling pathway (GO:0050853)COP9 signalosome (GO:0008180)COP9 signalosome (GO:0008180)COP9 signalosome (GO:0008180)Golgi apparatus (GO:0005794)Grb2-EGFR complex (GO:0070436)RNA binding (GO:0003723)Ras protein signal transduction (GO:0007265)Ras protein signal transduction (GO:0007265)SH3 domain binding (GO:0017124)Schwann cell development (GO:0014044)T cell activation (GO:0042110)actin cytoskeleton organization (GO:0030036)cell cortex (GO:0005938)cell-cell junction (GO:0005911)cellular response to ionizing radiation (GO:0071479)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytosol (GO:0005829)cytosol (GO:0005829)cytosol (GO:0005829)endosome (GO:0005768)endosome (GO:0005768)ephrin receptor binding (GO:0046875)ephrin receptor binding (GO:0046875)epidermal growth factor receptor binding (GO:0005154)epidermal growth factor receptor binding (GO:0005154)epidermal growth factor receptor signaling pathway (GO:0007173)epidermal growth factor receptor signaling pathway (GO:0007173)epidermal growth factor receptor signaling pathway (GO:0007173)extracellular exosome (GO:0070062)guanyl-nucleotide exchange factor adaptor activity (GO:0005091)guanyl-nucleotide exchange factor adaptor activity (GO:0005091)identical protein binding (GO:0042802)insulin receptor signaling pathway (GO:0008286)insulin receptor signaling pathway (GO:0008286)insulin receptor substrate binding (GO:0043560)insulin-like growth factor receptor signaling pathway (GO:0048009)membrane (GO:0016020)myelination (GO:0042552)natural killer cell mediated cytotoxicity (GO:0042267)negative regulation of natural killer cell mediated cytotoxicity (GO:0045953)neurotrophin TRKA receptor binding (GO:0005168)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)phosphotyrosine residue binding (GO:0001784)phosphotyrosine residue binding (GO:0001784)plasma membrane (GO:0005886)plasma membrane (GO:0005886)plasma membrane (GO:0005886)positive regulation of Rac protein signal transduction (GO:0035022)positive regulation of reactive oxygen species metabolic process (GO:2000379)protein binding (GO:0005515)protein domain specific binding (GO:0019904)protein kinase binding (GO:0019901)protein phosphatase binding (GO:0019903)protein-macromolecule adaptor activity (GO:0030674)protein-macromolecule adaptor activity (GO:0030674)receptor internalization (GO:0031623)regulation of MAPK cascade (GO:0043408)signal transduction (GO:0007165)signal transduction in response to DNA damage (GO:0042770)transmembrane receptor protein tyrosine kinase adaptor activity (GO:0005068)vesicle membrane (GO:0012506)
Expression (TPM)
GRB2 — as a Regulated Gene

TFs regulating GRB2 0 TFs

Transcription factors with Perturb-seq knockdown data for GRB2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GRB2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to GRB2

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GRB2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr17:75,104,838–75,106,094 299.9 kb Distal (>10kb) Multiome 454
chr17:75,109,401–75,110,734 295.8 kb Distal (>10kb) Multiome 762
chr17:75,113,757–75,114,535 291.5 kb Distal (>10kb) Multiome 585
chr17:75,130,577–75,132,090 274.0 kb Distal (>10kb) Multiome 990
chr17:75,153,354–75,155,273 251.1 kb Distal (>10kb) Multiome 993
chr17:75,182,199–75,183,441 222.7 kb Distal (>10kb) Multiome 908
chr17:75,205,060–75,205,995 200.2 kb Distal (>10kb) Multiome 975
chr17:75,240,373–75,240,941 165.0 kb Distal (>10kb) Multiome 436
chr17:75,261,304–75,262,404 144.0 kb Distal (>10kb) Multiome HiCAR 983
chr17:75,270,231–75,271,942 134.4 kb Distal (>10kb) Multiome HiCAR 946
chr17:75,288,761–75,289,874 116.2 kb Distal (>10kb) Multiome 941
chr17:75,346,809–75,347,324 58.5 kb Distal (>10kb) Multiome 265
chr17:75,393,479–75,394,300 11.8 kb Distal (>10kb) Multiome 834
chr17:75,404,856–75,406,161 81 bp At TSS Multiome 917
chr17:75,412,685–75,412,890 7.0 kb Proximal (<10kb) 414
chr17:75,456,149–75,457,264 50.9 kb Distal (>10kb) Multiome 865
chr17:75,481,081–75,481,680 75.7 kb Distal (>10kb) Multiome 64
chr17:75,514,425–75,517,171 110.0 kb Distal (>10kb) Multiome 1158
chr17:75,524,764–75,527,160 119.8 kb Distal (>10kb) Multiome 951
chr17:75,549,465–75,550,388 144.3 kb Distal (>10kb) Multiome 602
chr17:75,587,697–75,590,735 183.7 kb Distal (>10kb) Multiome 744
chr17:75,633,694–75,634,328 228.4 kb Distal (>10kb) Multiome 559
chr17:75,666,763–75,667,767 261.6 kb Distal (>10kb) Multiome 783

Genome Browser

Genomic view of the GRB2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr17:75,094,838 – 75,677,767
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq
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