Predicted to enable Arp2/3 complex binding activity. Predicted to be involved in actin filament debranching and negative regulation of Arp2/3 complex-mediated actin nucleation. Predicted to act upstream of or within learning and locomotory behavior. Predicted to be active in cortical actin cytoskeleton. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for GMFB. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GMFB upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GMFB, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:54,291,237–54,292,121 | 197.4 kb | Distal (>10kb) Multiome | 321 | |
| chr14:54,361,880–54,362,724 | 126.7 kb | Distal (>10kb) Multiome | 146 | |
| chr14:54,366,777–54,367,506 | 122.0 kb | Distal (>10kb) Multiome | 393 | |
| chr14:54,396,464–54,397,605 | 92.1 kb | Distal (>10kb) Multiome | 808 | |
| chr14:54,440,764–54,441,825 | 47.8 kb | Distal (>10kb) Multiome | 860 | |
| chr14:54,472,369–54,472,855 | 16.4 kb | Distal (>10kb) Multiome | 154 | |
| chr14:54,488,387–54,489,472 | 67 bp | At TSS Multiome | 922 | |
| chr14:54,490,285–54,490,461 | 1.3 kb | Proximal (<10kb) | 55 | |
| chr14:54,490,562–54,490,737 | 1.5 kb | Proximal (<10kb) | 72 | |
| chr14:54,493,438–54,493,627 | 4.4 kb | Proximal (<10kb) | 55 | |
| chr14:54,509,366–54,510,519 | 20.8 kb | Distal (>10kb) Multiome | 801 | |
| chr14:54,564,898–54,567,596 | 76.3 kb | Distal (>10kb) Multiome | 963 | |
| chr14:54,567,716–54,568,327 | 79.1 kb | Distal (>10kb) Multiome | 296 | |
| chr14:54,773,202–54,773,766 | 284.3 kb | Distal (>10kb) Multiome | 548 |
Genomic view of the GMFB locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.